standard human ige  (Bio-Rad)

Journal: Gut Microbes

Article Title: Immunogenicity and cross-protective efficacy induced by delayed attenuated Salmonella with regulated length of lipopolysaccharide in mice

doi: 10.1080/19490976.2024.2424983

Figure Lengend Snippet: Immune response to OMPs from S . Typhimurium induced by the regulated delayed attenuated Salmonella .Serum and vaginal secretions of mice were gathered at week 8 post-initial vaccination. Quantitative ELISA was applied to analyze the levels of specific IgG (a), IgA (b), IgG1 (c), and IgG2a (d) against S . Typhimurium OMPs. The results displayed the precise levels of antibodies, as measured by a standard curve, in mice orally inoculated with the regulated delayed attenuated Salmonella at the scheduled weeks. The standard differences between the mice in each group were shown by the error bars. Data are presented as the means ± SEM ( n = 8), superscript letters a, b, and c indicate p < 0.05 for comparisons with the BSG, SW112 ( waaG ), and SW116 ( waaJ ) groups, respectively.

Article Snippet: To establish a standard curve for each antibody isotype, the mouse Ig standard (1010–01, Southern Biotech, USA) was coated onto the last two rows of each plate at a concentration of 200 ng/100 μl overnight under a temperature around 4°C.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Gut Microbes

Article Title: Immunogenicity and cross-protective efficacy induced by delayed attenuated Salmonella with regulated length of lipopolysaccharide in mice

doi: 10.1080/19490976.2024.2424983

Figure Lengend Snippet: Immune response to OMPs from S . Choleraesuis and S . Enteritidis induced by the regulated delayed attenuated Salmonella .Serum and vaginal secretions of mice were gathered at week 8 post-initial vaccination. Quantitative ELISA was applied to analyze the levels of specific IgG (a, c) and IgA (b, d) against OMPs from S . Enteritidis and S . Choleraesuis, as well as the subtypes IgG1 (e, g) and IgG2a (f, h). The results displayed the precise levels of antibodies, as measured by a standard curve, in mice orally inoculated with the regulated delayed attenuated Salmonella at the scheduled weeks. The standard differences between the mice in each group were shown by the error bars. Data are presented as the means ± SEM ( n = 8), superscript letters a, b, and c indicate p < 0.05 for comparisons with the BSG, SW114 ( waaI ), and SW120 ( wbaP ) groups, respectively.

Article Snippet: To establish a standard curve for each antibody isotype, the mouse Ig standard (1010–01, Southern Biotech, USA) was coated onto the last two rows of each plate at a concentration of 200 ng/100 μl overnight under a temperature around 4°C.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Gut Microbes

Article Title: Immunogenicity and cross-protective efficacy induced by delayed attenuated Salmonella with regulated length of lipopolysaccharide in mice

doi: 10.1080/19490976.2024.2424983

Figure Lengend Snippet: Immune response to OMPs from other enteric bacteria induced by the regulated delayed attenuated Salmonella .The sera were gathered from mice in SW114 ( waaI ), SW116 ( waaJ ), and SW118 ( waaL ) groups on days 42 after the primary immunization. Quantitative ELISA was applied to analyze the levels of specific IgG against OMPs from E. coli O8 (A), E. coli O138 (B), and E. coli O139 strain (C). The results displayed the precise levels of antibodies, as measured by a standard curve. The standard differences between the mice in each group were shown by the error bars. Data are presented as the means ± SEM ( n = 8), superscript letters a indicate p < 0.05 for comparisons with the BSG groups.

Article Snippet: To establish a standard curve for each antibody isotype, the mouse Ig standard (1010–01, Southern Biotech, USA) was coated onto the last two rows of each plate at a concentration of 200 ng/100 μl overnight under a temperature around 4°C.

Techniques: Bacteria, Enzyme-linked Immunosorbent Assay

Journal: Clinical and Translational Allergy

Article Title: Deep analysis of immune response and metabolic signature in children with food protein induced enterocolitis to cow’s milk

doi: 10.1186/s13601-018-0224-9

Figure Lengend Snippet: Analysis of proliferative T cells in CMA patients after allergen reactivation. PBMC from IgE-CMA ( a ) or FPIES-CMA ( b ) patients were labelled with CFSE and then cultured for 6 days with PBS or allergens purified from cow’s milk. Cells were then recovered and labelled with anti-human CD4. Among SSC-FSC gated cells, single cells were selected and analyzed for CD4 expression and CFSE signal. Proliferative T cells are defined as CD4 + CFSE low cells within a selected population (red square). Proliferative cells after reactivation with PBS, BLG, caseins or \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upalpha$$\end{document} α -lact are shown

Article Snippet: Standard curves obtained with anti-human IgE [ ] or anti-human IgG (F(ab′)2 Fragment specific, Pierce ® , Thermo Scientific, Rockford, USA) coated plates and standard human IgE (World Health Organization; concentrations ranging from 10 to 0.08 IU/mL) or commercial isotype standard IgGs (all from AbD-Serotec, Bio-Rad, concentrations ranging from 1 μg/mL to 50 ng/mL) were used as reference to quantify antibody concentrations.

Techniques: Cell Culture, Purification, Expressing